Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Expert Opin Drug Deliv. (2012). Curley SA. To overcome these limitations, microporation devices that act as therapeutic vehicles for delivering drug molecules across the skin barriers have been developed. Carbohydrate Polymers 88 373-381. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Clipboard, Search History, and several other advanced features are temporarily unavailable. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. Microporation was performed 2, 5, and 10 times on each sample. Masuelli, M. A. Journal of Polymer Science, 17, 527-546. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. ; Veldhuis, T.F.J. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Dilute Solution. Dextran An official website of the United States government. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Webdextran hydrodynamic radius. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Carbohydrate Polymers, 74, 372-378. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. ; Wood, P.J. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free 1984;17(6):11703. The unfolding of iRFP713 in a crowded milieu - PeerJ Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Google Scholar. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. Learn More. Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. Bahary, W.S. Journal of Applied Polymer Science, 13, 2031-2032. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Journal of American Chemical Society, 64(11), 2716-2718. Differences were considered statistically significant at p <0.05. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Ficoll and dextran vs. globular proteins as probes for testing Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Article FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). (1954). In fact, short chains do contain minor branching points. Human skins (Full-thickness human skin) were supplied by HansBiomed. If there are particles in the sample, the incident laser light gets scattered in all directions. Solution properties of branched dextran. The slope is a value and intercept is equal to the ln kMH value [47]. Advanced Drug Delivery Reviews, 60, 1650-1662. Masuelli, Martin Alberto. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. "Dextrans in Aqueous Solution. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Soc., 16, 3A, 426-433. Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. High Manufacturing Standards of Molecular Probes Dextrans. Epub 2022 Feb 2. Dextrans can function efficiently as anterograde or retrograde tracers, The Transdermal diffusion cell system was protected from light. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. Journal of Applied Polymer Science, 72, 871-876. Carbohydrate Polymers 72, 356-360. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t 2019;9(3-S):9726. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Microporation techniques for enhanced delivery of therapeutic agents. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Gold MH, Biron JA. Journal of Physical Chemistry. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. What is hydrodynamic radius Part of Chen Rong Huei; Min Lang Tsai (1998). These studies were carried out at the Hisko (Gunpo, Korea). Garcia de la Torre, J.; Carrasco, B. Springer Nature. Ortega, A.; Garca de la Torre, J. Carbohydr Polym. Viscosity of Liquids. Eng. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Dextran Fractions. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. The effect of branching is best recognized from the molecular weight dependencies of []. The Mark-Houwink a constant is close to 0.5 in poor solvents. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient.
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